|
KMID : 1134820070360040431
|
|
Journal of the Korean Society of Food Science and Nutrition
2007 Volume.36 No. 4 p.431 ~ p.438
|
|
|
Inhibitory Effect of the Methanolic Extract of Symphyocladia latiuscula on the Growth of HT?29 Human Colon Cancer Cells
|
|
Kim Eun-Ji
Park So-Young
Hong Ji-Eun
Shin Min-Jeong
Lim Soon-Sung
Shin Hyun-Kyung
Yoon Jung-Han
|
|
|
Abstract
|
|
|
|
In the present study, twenty eight marine algae species were evaluated for their antiproliferative effect on HT-29 human colon cancer cells. Among these, the methanolic extract of Symphyocladia latiuscula (SL Ex) showed the highest inhibitory activity on HT-29 cell growth. In this study, we examined the mechanism by which SL Ex inhibited the HT-29 cell growth. Cells were cultured with various concentrations of (0～20 ㎍/mL) SL Ex. The SL Ex substantially decreased the viable cell numbers and induced apoptosis of HT-29 cells in a dose-dependent manner. Western blot analyses of total cell lysates revealed that SL Ex increased the levels of cleaved caspase-8, -9, -7, and -3, and poly (ADP-ribose) polymerase in HT-29 cells. In addition, SL Ex increased truncated Bid levels but moderately decreased Bax levels at only 20 ㎍/mL. Furthermore, SL Ex did not affect Bcl-2 protein levels but increased the levels of Fas in HT-29 cells. The present results indicate that SL Ex inhibits cell growth via inducing apoptosis in human colon cancer cells. The mechanism of apoptosis induction by SL Ex involves caspase-8 activation leading to changes in mitochondrial events and subsequent activation of the caspase-7/caspase-3 cascade. Our finding may lead to the development of new therapeutic strategies for the treatment of colon cancer.
|
|
|
KEYWORD
|
|
|
Symphyocladia latiuscula, colon cancer, apoptosis, caspase, truncated Bid
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|